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Cytiva Europe
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Danaher Inc
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Biacore
cm7 sensor chip ![]() Cm7 Sensor Chip, supplied by Biacore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/cm7+sensor+chip+flow+chambers/chips+cm7+sensor/pm40723395-147-1-5 Average 86 stars, based on 1 article reviews
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Cytiva Europe
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BioCore Inc
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BioCore Inc
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NanoTemper Technologies
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Danaher Inc
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Cytiva Europe
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Danaher Inc
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Image Search Results
Journal: Cell Metabolism
Article Title: Imatinib and methazolamide ameliorate COVID-19-induced metabolic complications via elevating ACE2 enzymatic activity and inhibiting viral entry
doi: 10.1016/j.cmet.2022.01.008
Figure Lengend Snippet:
Article Snippet: The SPR assays were performed to analyze the interactions between the compounds and ACE2 protein (Sino Biological, China) by using a Biacore T100 machine with
Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, RNA Extraction, SYBR Green Assay, Activity Assay, Cholesterol Assay, Software
Journal: Viruses
Article Title: Phenothiazines Inhibit SARS-CoV-2 Entry through Targeting Spike Protein
doi: 10.3390/v15081666
Figure Lengend Snippet: Phenothiazines bound to the spike of SARS-CoV-2 and inhibited its cleavage. ( A – E ) Purified SARS-CoV-2 S proteins were immobilized on the CM7 sensor chip, and the candidate compounds including perphenazine ( A ), fluphenazine decanoate ( B ), acepromazine maleate ( C ), prochlorperazine maleate ( D ), and alimemazine hemitartrate ( E ) with different concentrations (from 7.18 to 250 μM) flowed over it for the SPR. The K D values were analyzed by Biocore Insight Evaluation Software. ( F ) The inhibitory activities of phenothiazines on CTSL-mediated S protein cleavage. Purified CTSL protein was incubated with or without candidate compounds on ice for 15 min, then S protein was added and incubated at 37 °C for 1 h. Proteins were subjected to silver staining. The expression of the full-length protein was quantitated by measuring band intensities using ImageJ software (version 1). Teicoplanin was used as a positive control. ( G , H ) Prochlorperazine maleate or teicoplanin was co-incubated with SARS-CoV-2 PsV at 37 °C for 1 h prior to being added to the HEK293T-hACE2 cells. The spike protein level was detected by Western blotting ( G ). The expression of the full-length protein was quantitated by measuring band intensities using ImageJ software ( H ). The values were normalized to β-actin. Data are presented as means ± SD. p -values below 0.05 ( p < 0.05) were considered to demonstrate statistically significant differences. Statistical significance was defined as * p < 0.05, ** p < 0.01, *** p < 0.001.
Article Snippet: Briefly, SARS-CoV-2 S protein (flow cell 2) was immobilized onto a
Techniques: Purification, Software, Incubation, Silver Staining, Expressing, Positive Control, Western Blot